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Thursday, 12 March 2015

Viral Infection in Oral Cavity

                     


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  INTRODUCTION

Virus is a Latin word which means venom or poison. The size of the viruses varies in millimicrons. It consist of central core of DNA or RNA.Virally infected cells produce the same type of nucleic acid so susceptibility of cells infection may be increased. Viruses cause certain infectious diseases and produce long-lasting immunity against reinfection by the same virus.
In addition , there are many neoplasm can be caused due to the viral infection. The classification of virus is difficult because of size of viruses and their incompletely understood metabolic systems. However, their classification based on biologic, chemical and physical properties of the animal viruses, separating them into groups according to the type of nucleic acid, and the size, shape and substructure of particle has been undertaken by the International Committee on Nomenclature of Viruses of the International Association of Microbiological Societies.



CLASSIFICATION OF MAJOR VIRUS GROUPS AND DISEASE
                                               
                                                  VIRAL LESIONS VIRUS
 


 DNA   VIRUS                                                                                                  RNA     VIRUS
A)      Herpes Virus                          
      1)  Herpes Simplex    
      2)  Varicella/ herpes zoster
      3)   Cytomegalic/ inclusion disease
      4)  Epstein- Barr Virus        
     Infectious Mononucleosis
B)      Pox Virus                      
1)  Small Pox                      
2)  Molluscum Contagiosm 
C)      Adeno Virus                           
1) Pharyngo Conjunctival Fever
2) Epidemic Kerato conjuntivitis
D)      Parvo Virus                            
E)      Irido Virus                    
F)      Papova Virus                
              1)Human Warts or Papillomas                      
              2) Tumorogenic Viruses
A)      Orthomyxo Virus                               
               1) Influenza
B)      Paramyxo Virus
                1) Measles
                2) Mumps
C)      Rhabdo Virus
                1) Rabies
                2) Hemorrhagic fever
D)      Arena  Virus
                1) Lymphocytic Choriomeningitis
                2) Lassa Fever
E)      Calci Virus
F)      Coona Virus
                 1)     Upper Respiratory Infection
G)      Bunya Virus
H)      Picorna Virus
                1) Poliomyelitis
                2) Coxsackie disease
                3) Common Cold
                4) Foot & Mouth Disease
                5) Encephalomyocarditis
I)       Reo Virus
T)      Toga Virus
                1) Rubella
                 2) Yellow fever
                3) St. Lovis Encephalitis
K)      Retro Virus
    ( RNA Tumour Virus  )
                    


 HERPES SIMPLEX INFECTION

 The Herpes simplex virus (HSV) – DNA virus. It bbelongs to human herpes virus (HHV) family, also called Herpetoviridae. Other members of this family are varicella-zoster virus, Epstein-Barr virus, Cytomegalovirus etc. Humans  are only known natural reservoirs and can stay in the host for life and become periodically reactivated.
TYPES OF HSV
·     Herpes simplex virus – 1:  This spread through saliva. The lesions above the waist, in oral, facial and ocular areas including pharynx, and skin.
·     Herpes simplex virus – 2: This transmitted through sexual contact. It involves genitalia and skin below the waist.
    PATHOGENESIS
Herpes Simplex virus infections are seen  in patterns . They are :
·      Primary infection: -This is initial exposure to virus, no   antibodies are produced. It commonly occurs in young age, often  asymptomatic. The virus taken up in sensory nerves and transported to associated ganglia.
·     Secondary infection: - It also known as recurrent infection. The virus can  residing in ganglia reactivated.  It is not always symptomatic; sometimes patients only shed the virus through saliva.
The predisposing factors for reactivation of virus are old age, UV light, emotional stress, trauma, menstruation, systemic diseases or malignancy .

     PRIMARY HERPETIC GINGIVOSTOMATITIS
The primary herpetic gingivostomatitis is a relatively common viral infection of the oral mucosa. The herpes simplex virus could be isolated from patients with a particular clinical configurations.
 The etiology for primary herpetic gingivostomatitis are Herpes simplex   virus type 1  and Herpes simplex virus type 2 .
  
CLINICAL  FEATURES  
       The age incidence of this viral infection is  6 months – 5 years . The sex incidence is nil. The site predilection are affects movable and immovable mucosa , primarily gingiva, labial mucosa, vermilion zone, palate. The onset of the disease is abrupt.
         Primary herpetic gingivostomatitis is clinically characterized by high fever ,headache, malaise, anorexia ,irritability ,bilateral sensitive regional lymphadenopathy ,sore mouth lesions. The affected mucosa is red and edematous, with numerous coalescing vesicles, which rapidly rupture, leaving painful small, round, shallow ulcers covered by yellow fibrin New lesions continue to develop during the first three to five days. The ulcers heal in 10–14 days. Both the movable and non movable oral mucosa may be affected. Gingival lesions are almost always present, resulting in enlargement and edematous and painful erosions. The diagnosis is usually made on clinical grounds.
                              
                   Primary herpetic gingivostomatitis: multiple ulcers on the tongue
LABORATORY TESTS
Viral isolation from tissue culture inoculated with the fluid of in tact vesicles is the most definitive diagnostic procedure.  The serologic tests for HSV antibodies are positive 8 days after the initial exposure. These antibody titres are useful in documenting past exposure and are used primarily in epidemiologic studies. Two of the most commonly used diagnostic procedures are the cytologic smear and tissue biopsy, with cytologic study being the least invasive and most cost effective.
DIFFERENTIAL DIAGNOSIS
        Aphthous ulcers, hand-foot-and-mouth disease, herpangina, acute necrotizing ulcerative gingivitis, erythema multiforme, early pemphigus, desquamative gingivitis.
TREATMENT
        Mainly symptomatic treatment and in severe cases the systemic  Acyclovir or Valaciclovir can be given.

       SECONDARY HERPETIC STOMATITIS
        Recurrent herpetic stomatitis is usually seen in adult patient and manifests itself clinically as an attempted form of the primary lesion.
ETIOLOGY
        The secondary or recurrent herpetic stomatitis is a relatively common oral and perioral disease that is due to reactivation of HSV1. It is commonly precipitated by fever, trauma, cold, heat, sunlight, emotional stress, and HIV infection.

                    
                Secondary herpetic stomatitis: small round ulcers on the palate

CLINICAL FEATURES
The most common sites of recurrence for HSV-1 are lips and perioral skin , palate, attached gingiva. Clinically, the lesions present as multiple small vesicles arranged in clusters .The vesicles soon rupture, leaving small ulcers that heal spontaneously within 6–10 days. The prodromal symptoms are burning, itching, tingling, and erythema. Characteristically, fever, generalized regional lymphadenopathy ,and constitutional symptoms are absent. The diagnosis is made on clinical grounds.
DIFFERENTIAL DIAGNOSIS
        Aphthous ulcer, primary and secondary syphilis, streptococcal stomatitis, herpangina .
TREATMENT
          The symptomatic treatment is required.
               
               HERPETIC WHITLOW
        This are less common presentation , mainly infection of fingers, and thumb. It occurs due to self inoculation in children with orofacial HSV. It can also occur in medical and dental professionals who do not wear gloves and come in contact with infected patients.

                  HERPANGINA
The herpangina is an acute self-limiting viral infection, also known as aphthous pharangitis or vesicular pharangitis . Most cases arise in the summer or early fall in nontropical areas  with crowding and poor hygiene aiding their spread. The faecal-oral route is considered the major path of transmission, and frequent hand washing is emphasized in an attempt to diminish spread during epidemics.
ETIOLOGY
      Usually caused by coxsackievirus group A, types 1–6, 8, 10, and 22, and less commonly by other types.
CLINICAL FEATURES
       The age incidence are  commonly in children , occasionally in adult The  incubation period  of 2-10 days The disease presents with an acute onset of fever, sore throat,  dysphagia, headache, and malaise, followed by diffuse erythema and vesicles. The vesicles are small and numerous, and rupture rapidly, leaving painful ulcers that heal within 7–10 days .
ORAL MANIFESTATION
        Characteristically, the lesions appear on the soft palate and uvula, tonsillar pillars, and posterior pharyngeal wall.

DIFFERENTIAL DIAGNOSIS
         The herpetiform ulcers, aphthous ulcers, primary herpes simplex infection, acute lymphonodular pharangitis, erythema multiforme, FAPA syndrome, hand-foot-and-mouth disease.
TREATMENT
          The supportive treatment is needed.
  

   ACUTE LYMPHONODULAR PHARYNGITIS
      The acute lymphonodular pharangitis is an acute febrile disease caused by strains of coxsackie virus A 10. It marked resemblance with herpangina .
CLINICAL FEATURES
          This viral disease affect mainly in children and young adult. The incubation period of  2-10 days. The chief complaints are sore throat, an elevation of temperature, mild headache, anorexia.
ORAL MANIFESTATION
The raised , discrete , whitish or yellowish to dark pink solid papules or nodules can be seen. It is surrounded by narrow zone of erythema. The main sites are uvula, soft palate, anterior pillars, posterior oropharynx .                    
LABORATORY FINDINGS
The primary isolation of Coxsackie A10 Virus is main laboratory finding.
HISTOLOGIC FEATURES
The hyperplastic lymphoid aggregates in papules or nodules can seen.
TREATMENT
No treatment required for this viral infection.


         HAND-FOOT-AND-MOUTH DISEASE

Hand-foot-and-mouth disease is an acute self-limiting contagious viral infection transmitted from one individual to another. 
ETIOLOGY
       The main etiology is Coxsackievirus strain A16, and rarely other strains.
CLINICAL FEATURES
The disease usually affects children and young adults, and often occurs in epidemics. Skin lesions are not constant, and present as small vesicles with a narrow red halo. The lateral borders and the dorsal surfaces of the fingers and toes are the most common areas involved. The lesions may appear on the palms, soles, and buttocks. The disease lasts five to eight days.
ORAL MANIFESTATIONS
 It characterized by small vesicles and 5–30 in number. It rapidly rupture, and leaving painful, shallow ulcers with diameter 2–6 mm and they are surrounded by a red halo. The buccal mucosa, tongue, and labial mucosa are the most commonly affected sites. The diagnosis is made on the basis of clinical criteria.
                   
Hand-foot-and-mouth disease: shallow ulcers on the buccal mucosa.
DIFFERENTIAL DIAGNOSIS
The aphthous ulcers, herpes simplex infection, herpangina are differential diagnosis.
TREATMENT
 The supportive treatment is needed.

       FOOT AND MOUTH DISEASE
The viral infection which rarely affect man, but does affect dogs and sheep as well as cattle. The  transmission of disease through contact with infected animals.
CLINICAL  FEATURES  
Fever , nausea , vomiting , malaise are main clinical features.
ORAL MANIFESTATION
The ulcerative lesion of oral mucosa. The lesion begins as small vesicle and rupture and heals within 2 weeks

                                MEASLES
Measles is an acute highly contagious viral disease caused by measles virus. It is characterized by fever, URT catarrhal inflammation,  koplik’s spots and maculopapules. The disease may complicated with branch- pneumonia, encepholitis, hepatitis.
ETIOLOGY
Pathogen is measles virus. it  has been classed as a paramyxo virus. The site of  the measles virus exists  measles can be detected from blood and  nasal,  pharyngeal secretions.

EPIDEMIOLOGY
The patients are  the only source of infection. The air-borne is the routes of transmission. All age person is susceptible90% of contact people acquire the disease. The permanent  immunity acquired after disease.The winter and spring season are main season for this viral infection.
CLINICAL  FEATURES
The incubation period  of  8-10 days. The prodromal phase of infection is 3 to 4 days. The clinical features during prodromal phases are fever, malaise, catarrhal inflammation of URT ,Koplik’s   spots, transient prodromal rashes. The temperature rise continuously during this time
The Eruption stage is appear 3-5 days after fever. The rashes are maculopapular in nature.
   Sequence: Behind the ear→along the hairline → face → neck →     chest →   back → abdomen → limbs  → hand and feet
            During Convalescent  stage  brown staining , fine branny desquamation   can seen.
ORAL MANIFESTATION
Koplik's spot  is a intraoral characteristic small spot, are small irregularly shaped bluish white speck surrounded by bright red margin. It is usually occur in buccal mucosa due to immune reaction to virus in endothelial cells of dermal capillaries.
The palatal and pharyngeal petechiae ,generalized inflammation, congestion and swelling, focal ulceration of gingiva, palate and throat are the other manifestations.
                          Koplik’s spot in Measles
COMPLICATIONS
 The complications are bronchopneumonia, myocarditis, laryngitis, neurologic complications like encephalitis, otitis media, noma.
DIFFERENTIAL DIAGNOSIS
The rubella (German measles)  , roseola infantum , drug  rashes are the differential diagnosis.
TREATMENT
 The general  therapy are  rest, nursing and diet . The symptomatic therapy  for fever and cough. Then treat the complications of  measles.
PREVENTION
 The best method of prevention is control the source of infection, interruption of transmissions, protection of the susceptible person. The active immunization by MMR vaccine is best way of prevention. Passive immunization  like placenta globulin or gamma globulin mainly given for relieve symptoms.       
                    RUBELLA
     Rubella is a mild viral illness that is produced by Toga virus. The virus is contracted through respiratory droplets. The incubation time is from 14 to 21 days , and infected patients are contagious during this period. From 1 week before the exanthem to about 5 days after the development of the rash. But infants with a congenital rubella affected.
CLINICAL FEATURES
Prodromal symptoms may be seen 1 to 5 days before the exanthem  include fever, headache , malaise, anorexia, myalgia , mild conjunctivitis , coryza, pharangitis, cough, and lymphadenopathy.   The complication are arthritis , rash , encephalitis , thrombocytopenia. The oral lesions are small discrete dark-red papules that develop on the soft palate and may extend onto the hard palate.
TREATMENT
 The Nonaspirin antipyretics and antipruritic medications given. The passive immunity  with the administration of human rubella immunoglobulin given to relieve symptoms.
PREVENTION
The active immunization can be done with MMR vaccine.
                        
           SMALL POX
The smallpox is an acute viral disease, which was epidemic in  nature and very fatal before the discovery of vaccine.
CLINICAL FEATURE
The incubation period of smallpox is 7-10 days. It manifests with high fever,nausea,vomitted,chills and headache. The skin lesions as small macules and papules appearing first on the face and then spreading rapidly to the rest of the body. The papules develop into vesicles which then become pustules and they are small, elevated and yellowish green with an inflamed border, which gets infected secondarily and become hemorrhagic. The desquamation occur  when  healing begins. The common complication is severe pitting or pocking of the skin as a result of pustule formation.
ORAL  MANIFESTATIONS
   The ulceration of the oral mucosa and pharynx with multiple vesicle formation which rupture and form non specific ulcer. And the tongue is swollen and painful making swallowing difficult.
COMPLICATION
 The main complications are abscess formation with septicemia, respiratory infection, erysipelas,  eye infection and ear infection

       MOLLUSCUM  CONTAGIOSUM
It is a pox virus. It occur on skin and mucosal surfaces. It is also consider tumour like due to the typical  localised epithelial proliferation caused by the virus
CLINICAL FEATURES
 This viral lesion is commonly seen  in children and young adults. The single  or multiple discrete elevated nodules occurs on the arms, legs , trunk , face and particularly eyelids. This infection can sexually transmitted or spread by autoinoculation. The incubation period is 14-50 days. The lesions are hemispheric in shape with a central umblication which may be keratinized. The oral lesion may occur on lips, tongue and buccal mucosa
HISTOLOGIC FEATURES
Thickening and down growth of the epithelium with the formation of large eosinophilic intracytoplasmic inclusion bodies  known as  “Henderson-petterson inclusion” or  “molluscus bodies”
TREATMENT
· Surgical excision
· Topical application of podophyllin or canthardin


        CONDYLOMA ACUMINATUM
Condyloma acuminta is a infectious disease  caused by Human papilloma virus.
CLINICAL FEATURES
The condyloma acuminta is commonly seen in children. Soft  pink nodule can seen. It proliferate and coalesce rapidly to form diffuse papillomatous clusters of varying size.
ORAL MANIFESTATION
  The nodules are small, multiple, white or pink in colour. Papillomatous, bulbous masses scattered over tongue .
HISTOLOGIC FEATURES
 The parakerotic surface with marked underlying acanthosis. The vacuolated cells in spinous layer can seen. Supporting connective tissue is oedematous and dialated capilaries and chronic inflammatory cell infiltration is common. The intranuclear viral inclusion can be seen.
TREATMENT
The surgical excision is main treatment. And the topical podophyllin can be used.

                                           CHICKENPOX
 The chickenpox is a acute viral disease caused by  varicella zoster virus. It  represent the primary infection with varicella zoster virus. It spread through air droplets or direct contact with active lesion and portal of entry is respiratory tract.
CLINICAL FEATURES
  The main age incidence is between 5 – 9 years. The site predilection are face, trunk and extremities. The prodromal symptoms showssymptomatic phase of VZV infection begin with malaise, pharangitis, rhinitis.
The early vesicular stage is most characteristic of the lesion,
  the centrally located vesicle is surrounded by a zone of 
  erythema and described as “ a dew drops on a petal ”.
The lesion occur in successive crops over 3-4 days and lesions 
  in various stages are present at the same time oral lesion
  precede the skin lesions.
     
COMPLICATION
The encephalitis, pneumonia, Reye’s syndrome, spontaneous abortion or congenital defect can occur if the disease occur early pregnancy are main complications. In immunocomprimised persons extensive cutaneous involvement along with high fever, hepatitis, pneumonia , pancreatitis , encephlitis may occur.
HISTOLOGIC FEATURES
The main histologic features are acantholysis and formation of numerous free floating tzanck cells .
TREATMENT
    The anti viral drug like acyclovir  can be used for chickenpox.
                   
             HERPES ZOSTER
 The herpes zoster, or shingles, is an acute self-limiting viral disease of an extremly painful and incapacitating nature. It characterized by inflammation of dorsal root ganglia.



ETIOLOGY
 The etiology is reactivation of Varicella-zoster virus. The predisposing factors for reactivation of the virus are AIDS, leukemia, lymphoma, other malignancies, radiation, immunosuppressive and cytotoxic drugs ,old age.
CLINICAL FEATURES
The thoracic, cervical, trigeminal, and lumbosacral dermatomes are most commonly affected.  It is characteristically, one dermatome is usually affected.  Theprodromal symptoms are pain and tenderness ,headache ,pulpitis , malaise, fever . After two to four days, clusters of vesicles develop, and within two or three days evolve into pustules and ulcers, covered by crusts . The lesions persist for two to three weeks. The unilateral location of the lesions is a typical pattern of herpes zoster.
Oral manifestations occur when the second and third branches of the trigeminal nerve are involved. Post herpetic trigeminal neuralgia is a common complication, and rarely osteomyelitis, jawbone necrosis, and tooth loss are seen. The diagnosis is made on the basis of clinical criteria.
DIFFERENTIAL  DIAGNOSIS
 Herpes simplex,  erythema multiforme are the differential diagnosis.
                       MUMPS
 Mumps is an acute viral infection of the paramyxoviruses family. An acute onset of unilateral or bilateral tender, self-limited swelling of the parotid or other salivary gland lasting more than 2 days without other apparent cause. It can lead to brain inflammation, deafness or sterility.
 PATHOGENESIS 
 The transmission of virus is mainly respiratory tract.  Then virus can be replication in nasopharynx and regional lymph nodes This cause viraemia 12-25 days after exposure with spread to tissues. The multiple tissues infected during Viraemia
COMPLICATION
 The main complications are Epididymo orchitis may lead to atrophy, sterility, low sperm counts, pancreatitis,deafness, arthritis, oopharitis, nephritis, myocarditis. There is a CNS involvement in 60% cases may manifest with aseptic meningitis,encephalitis.
LABORATORY  DIAGNOSIS
No Laboratory confirmation needed typical cases. But for a typical infection needs laboratory Diagnosis. For that the virus isolated from saliva urine, CSF. The virus can be cculturing in Human amnion, He la cells. The other methods are immunoflourscence methods ELISA, Complement fixation test
       INFECTIOUS MONONUCLEOSIS
This  viral infection is also called Glandular fever. It results from exposure to Epstein-Barr virus (EBV). The transmission of virus through intimate contact like kissing, sharing of straws, contaminated salivary transmission in children. The exposure during childhood  is asymptomatic.  But it is symptomatic infections arise in young adults.
CLINICAL FEATURES
 The glandular fever mainly seen in children. The main site incidence are apart from systemic manifestations, affects anterior and posterior cervical chain lymph nodes as well as oropharyngeal tonsils. Intra orally lesion seen in hard palate and gingiva.
Signs & symptoms :
 The systemic signs and symptoms are fever, lymphadenopathy , pharangitis, rhinitis, cough, hepatosplenomegaly. In 90% cases, prominent, symmetric, tender enlargement of anterior and posterior cervical chain lymph nodes.
ORAL MANIFESTATION
Petechiae on hard or soft palate and enlargement of oropharyngeal tonsils.

DIAGNOSIS
The diagnosis is suggested by clinical presentation. The WBC count is raised with differential count showing lymphocytosis as high as 70% - 90%. The classical serological finding – presence of Paul – Bunnell heterophil antibody, present in 90% of affected patients.

ACQUIRED IMMUNODEFICIENCY SYNDROME                                                         (AIDS)
 More than 25 million cases  are present in worldwide. Considered almost 100% fatal and no known vaccine developed so far.
ROUTES OF TRANSMISSION
·          Sexual contact
·          Infected blood / blood products
·          Intravenous drug abuse
·          Transplacental transfer




PATHOGENESIS
When virus enters the body, its DNA incorporated into primary target cell i.e. CD4+ helper T lymphocyte. Similar to other viral infections, antibodies to virus are formed but are not protective.HIV virus can remain silent or cause cell death, as a result, decrease in helper T- cells occurs, leading to loss in immune function. There is an asymptomatic stage lasting for about 8 – 10 years after which the final symptomatic stage develops.
CLINICAL FEATURES
After inoculation, patient may be asymptomatic or develop acute response similar to infectious mononucleosis. The acute response  are fever, generalized lymphadenopathy, sore throat, myalgia, diarrhoea, maculopapular rash etc.
Acute syndrome clears within a few weeks and a variable asymptomatic phase follows which may last for 8 – 10 years.
Symptomatic phase shows opportunistic infections like  (pneumonia, CMV, HSV, TB etc) and neoplastic processes like (Kaposi sarcoma, Non-Hodgkin’s lymphoma etc).




ORAL MANIFESTATIONS
GROUP 1 (LESIONS STRONGLY ASSOCIATED WITH HIV)
1.       Oral candidal infections
·           Erythematous
·           Hyperplastic
·           Pseudomembranous
2.       Hairy leukoplakia
3.       HIV associated periodontitis
·           HIV gingivitis
·           HIV periodontitis
·           Necrotizing ulcerative gingivitis 
·           Necrotizing ulcerative stomatitis
4.       Kaposi sarcoma
5.       Non-Hodgkin’s lymphoma


             
     ORAL CANDIDIASIS                              HIV ASSOCIATED              HAIRY LEUKOPLAKIA
                                                                       PERIODONTITIS



      
             HIV associated gingivitis               Necrotizing ulcerative gingivitis      Necrotizing ulcerative stomatitis
 Stages of Kaposi sarcoma
               
           Patch stage                                               Plaque stage                                      Nodular stage

GROUP 2 (LESIONS LESS COMMONLY ASSOCIATED WITH HIV):
1.  Aphthous ulcers (oropharyngeal region)
2.  Idiopathic thrombocytopenia
3.  Salivary gland disorders
·     Dry mouth and decreased salivary flow
·     Uni or bilateral swelling of major glands
4.  Viral infections (apart from EBV)
·  Cytomegalovirus
·  Herpes simplex virus
·  Human papilloma virus
·  Varicella - zoster virus
             
HIV ASSOCIATED APHTHOUS ULCERS      HIV ASSOCIATED HPV INFECTION         HIV ASSOCIATED HERPETIC ULCERS

GROUP 3 ( LESION SEEN IN HIV INFECTION) ;
1.   Bacterial infection
·  Actinomyces Israeli
·  E  coli
·  Klebsiella pneumonia
·  Cat scratch disease
2.   Drug reaction
3.   Lichenoid reaction
4.   Epithelioid angiomatosis
5.   Fungal infection other than candidiasis
·Cryptococcus neoformans
·Histoplasma capsulatum
· Aspergilus flavus
6.   Neurologic disturbance
· Facial plasy
· Trigeminal neuralgia
7.   Recurrent aphthous stomatitis
8.   Viral infections
·     Cytomegalo virus
·     Molluscum contagiosum
DIAGNOSIS
1.     VIRAL BASED TESTS
·        Viral culture
·        PCR
·        P24 antigen detection : During window period and late
phase of infection
2.     ANTI-HIV ANTIBODY TESTS
·     Screening test: ELISA is most commonly used test.
                                                -But it can show false positive results.
·     Western Blot test: It is a test to detect viral antibodies.
                                                       -More accurate than ELISA.
3. IMMUNOLOGICAL MARKERS
·     CD4+ T cell count       :  Decreases
·     CD4/CD8 ratio             :  Reverse
 4. SALIVARY TESTS
5. GAC ELISA




TREATMENT
Highly active Antiretro viral therapy  is needed. Three types of medications are available The initial regimens consist of two nucleoside reverse  transcriptase inhibitors and one or two protease inhibitors. Alternatively , two nucleoside reverse transcriptase  inhibitors and a no nucleoside reverse transcriptase inhibitor can be used.
1.      Nucleoside reverse- transcriptase inhibitors
      Abacavir, didanosine, lamivudine, stavudine, zalcitabine or zidovudine
2. Nonnuc1eoside reverse transcriptase inhibitors
  Delavlrdlne,  efavirenz. or nevirapine
3. Protease inhibitors
 Amprenavir; indinavir; nelflnavh; ritonavir; or saquinavir T








                                     Reference

1.     Textbook of Oral pathology  6th edition               : Shafer


2.     Oral and maxillofacial Pathology  2nd edition       : Neville

3.     Textbook of Oral medicine                                      :  Anil Ghom


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